• Faculty


  • PerozichJ
  • Dr. John Perozich

    Professor of Biology

    113 Cosmas & Damian Hall
    (740) 284-5267
    jperozich@franciscan.edu
    Download Full CV

    Education

    • PhD. Biochemistry and Molecular Genetics - University of Pittsburgh School of Medicine (Pittsburgh, PA)
    • BS Biology major, Chemistry minor - Lebanon Valley College (Annville, PA)

    Professional Experience

    • Part-Time Adjunct Faculty. Department of Natural Sciences and Mathematics - Carlow College (Pittsburgh, PA)
    • Instructor in Bio-Organic Chemistry and Microbiology - Ohio Valley General Hospital School of Nursing (McKees Rocks, PA)
    • Teaching Assistant in Medical Microbiology and Biochemistry Conference - University of Pittsburgh School of Medicine (Pittsburgh, PA)

    Honors and Awards

    • 2011 Franciscan University of Steubenville Summer Faculty Development Award
    • 2010 Franciscan University of Steubenville Summer Faculty Development Award
    • 2008 FUS Faculty Enrichment Grant – Present Poster at OCCBIO 2008 Conference
    • 2008 Franciscan University of Steubenville Summer Faculty Development Award - Updating ALDH Alignment
    • 11th Edition of Who's Who Among American Teachers & EducatorsTM (2007)
    • Tenure – granted 8/2006
    • Promotion from Assistant to Associate Professor – 5/2004
    • 2002 Franciscan University of Steubenville Summer Faculty Development Award - Biochemistry Laboratory
    • 2001 Franciscan University of Steubenville Summer Faculty Development Award - Immunology Course

    Professional Memberships

    • American Association for the Advancement of Science (AAAS)
    • American Society for Microbiology (ASM)
    • International Society for Computational Biology (ISCB)
    • Protein Society

    Undergraduate Research Supervised

    Protein Bioinformatics: Supervised undergraduate research projects. Each student chooses a different protein to study. Protein sequences from various species or related proteins are accessed from databases and aligned. By analysis of the multiple sequence alignment and the tertiary structure, structural, functional and evolutionary relationships for the protein are elucidated.

    • Nicholas Freas. Fall 2013 – Spring 2014. Extended Analysis of Nucleotide Sugar Dehydrogenases.
    • Dorothy McAfee. Fall 2012 – Spring 2014. Comparison of Catechol-O-Methyltransferases with Other Class I Methyltransferases.
    • Moriah Holt. Fall 2012 – Spring 2013. Analysis of Clade A & B Serpins.
    • Josh Vanderhoof. Fall 2012. Analysis of RNases.
    • Michael Niemaszyk. Spring 2012 – Fall 2012. Comparison Sirtuin 2 to Other Histone Deacetylases.
    • Nicholas Cundiff. Spring 2012. Analysis of Pentapeptide Repeat Proteins.
    • Danielle Leatherby. Fall 2011 – Spring 2012. Comparison of Luciferases and other Adenylate-Forming Enzymes.
    • Katie Kirrane. Fall 2011. Analysis of Insulysin.
    • Christopher Maguire. Fall 2011. Analysis of Aromatic Amino Acid Hydroxylases.
    • Alyssa Morey. Fall 2011. Analysis of Pyruvate Kinases.
    • Anne McMahon. Spring 2011 - Fall 2011. Comparison of Influenza Neuraminadases to Other Sialidases.
    • Aaron Medina. Spring 2011. Analysis of Protein Phosphatase B.
    • Kathryn Steadham. Spring 2011. Analysis of the Chaperone protein GroEL.

    Research Interests

    My research interests focus on the enzyme Aldehyde Dehydrogenase (ALDH). ALDHs catalyze the oxidation of aldehydes to their corresponding carboxylic acids coupled with reduction of NAD or NADP to NADH or NADPH, respectively. ALDHs occur throughout all phyla of life. Many disparate aldehydes are ubiquitous in nature and most are toxic at low levels because of their chemical reactivity. Thus, levels of metabolic-intermediate and environmental aldehydes must be carefully regulated. For this, most well studied organisms are known to have several distinct ALDHs which take part in a variety of physiological roles. which is central to the detoxification of alcohol, xenobiotics and several chemotherapeutic agents in the human body. Through analysis of a large protein sequence alignment (145 sequences) by this investigator, a large number of important conserved amino acid residues were identified in the ALDH extended family. In collaboration with the University of Pittsburgh, the activity of these residues is being explored via site-directed mutagenesis of the rat class 3 ALDH, providing students with skills in molecular biology, protein biochemistry and enzyme kinetics.

    Another area of research interest is the growing field of bioinformatics. Modern sequence databases possess a large amount of untapped information. Student research projects involve performing multiple protein sequence alignments of certain proteins and/or protein families. From these alignments and comparisons to known tertiary structures, the identities and potential functions of conserved residues will be proposed. Also the evolutionary relationships between proteins from distinct species will be evaluated.

  • Dr. John Perozich - “Effect of Alignment Trimming on Protein Phylogenies: A Comparison of Trimming Methods. In preparation.” 
    Ropelewski AJ, Mendez RG, Perozich J, Wymore T, Nicholas HB. 2014. Effect of Alignment Trimming on Protein Phylogenies: A Comparison of Trimming Methods. In preparation.

    Dr. John Perozich- “Aldehyde dehydrogenase: Maintaining critical active site geometry at motif 8 in the class 3 enzyme. Eur J Biochem” 
    Aldehyde dehydrogenase: Maintaining critical active site geometry at motif 8 in the class 3 enzyme. Eur J Biochem

    Dr. John Perozich-An algorithm for identification and ranking of family-specific residues 
    An algorithm for identification and ranking of family-specific residues, applied to the ALDH3 family. Chem Biol Interact 143-144:23-28

    Dr. John Perozich-“Beyond the catalytic core of ALDH: A web important residues begins to emerge.” 
    Beyond the catalytic core of ALDH: A web important residues begins to emerge. Chem Biol Interact 130-132:39-46

    Dr. John Perozich-“Coenzyme specificity in aldehyde dehydrogenase.” 
    Coenzyme specificity in aldehyde dehydrogenase. Chem Biol Interact 130-132:115-124

    Dr. John Perozich- Comparative Analysis of Nucleotide-Sugar Dehydrogenases 
    Comparative Analysis of Nucleotide-Sugar Dehydrogenases. June 2009

    Dr. John Perozich-Comparison of Horse Prostate Kallikrein and Other Serine Proteases 
    Comparison of Horse Prostate Kallikrein and Other Serine Proteases. June 2008

    Dr. John Perozich-HSP90 N-Terminal Domain and its Role in the Binding and Hydrolysis of ATP 
    HSP90 N-Terminal Domain and its Role in the Binding and Hydrolysis of ATP. June 2009

    Dr. John Perozich-“Residue Conservations in Aldehyde Dehydrogenase Gene Fusion Products Reemphasize Functional Interpretations” 
    Residue Conservations in Aldehyde Dehydrogenase Gene Fusion Products Reemphasize Functional Interpretations. In: Enzymology and Molecular Biology of Carbonyl Metabolism 12, H Weiner, B Plapp, R Lindahl & E Maser, eds. West Lafayette IN: Purdue University Press, pp 8-14

    Dr. John Perozich-“Shifting the NAD/NADP preference in class 3 aldehyde dehydrogenase. Eur J Biochem” 
    Shifting the NAD/NADP preference in class 3 aldehyde dehydrogenase. Eur J Biochem

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